A targeted 3D EM and correlative microscopy method using SEM array tomography

Correlative Array Tomography – from 2D towards 3D

Correlative microscopy combines information from light and electron microscopy (LM and EM) into one comprehensive dataset. The “Shuttle & Find” interface from Zeiss was the first easy to use solution for imaging one and the same sample region in different microscopes, i.e. from widefield or confocal LMs to environmental or field emission SEMs (FE-SEM). However, Shuttle & Find is limited to 2D a...

Seeing a Mycobacterium-Infected Cell in Nanoscale 3D: Correlative Imaging by Light Microscopy and FIB/SEM Tomography

Mycobacteria pose a threat to the world health today, with pathogenic and opportunistic bacteria causing tuberculosis and non-tuberculous disease in large parts of the population. Much is still unknown about the interplay between bacteria and host during infection and disease, and more research is needed to meet the challenge of drug resistance and inefficient vaccines. This work establishes a ...

Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures

Nanomanipulation under scanning electron microscopy (SEM) enables direct interactions of a tool with a sample. We recently developed a nanomanipulation technique for the extraction and identification of DNA contained within sub-nuclear locations of a single cell nucleus. In nanomanipulation of sub-cellular structures, a key step is to identify targets of interest through correlating fluorescenc...

Projection Neuron Circuits Resolved Using Correlative Array Tomography

Assessment of three-dimensional morphological structure and synaptic connectivity is essential for a comprehensive understanding of neural processes controlling behavior. Different microscopy approaches have been proposed based on light microcopy (LM), electron microscopy (EM), or a combination of both. Correlative array tomography (CAT) is a technique in which arrays of ultrathin serial sectio...

Topics: 3D CLSM and Light Microscopy, 3D FIB/SEM or Serial Sectioning time resolved microscopy, 3D Image Analysis and simulation 3D TEM Tomography and Serial Sectioning, 3D X-ray Microscopy and Tomography, Ultra-Resolution Tomography Scientific committee and chairpersons: